Fig. 3. SM compounds degrade HIF-1α via the proteasome in hypoxia. a) HCT116 cells were treated with 25 µM 17-AAG, 25 µM SM compounds and/or the proteasome inhibitor MG262 (1 µM) for 6 hrs in hypoxia. b) Densitometry of cytoplasmic HIF-1α protein levels from three independent experiments. c) Densitometry of nuclear HIF-1α protein levels from three independent experiments.